Tag PCR

A new generation of technology, portable PCR, offers potential for affordable, immediate, pondside diagnosis in easy-to-use handheld kits. But will it live up to the hype? 

The University of Arizona Aquaculture Pathology Laboratory has carried out the Proficiency Test (PT) since 2005, with 300-plus diagnostic laboratories participating while improving their capabilities in the diagnosis of several shrimp pathogens. 

Indian shrimp hatcheries have experienced larval mortality in the zoea-2 stage, with molt deterioration and resulting in heavy mortality. Authors investigated the problem holistically. 

El sembrar postlarvas de camarón de la mejor calidad, sanas y libres de patógenos, es un paso crítico de gestión con efectos significativos en la producción y rentabilidad de una granja de camarón. 

Many shrimp producers give only perfunctory attention to routine biosecurity at hatcheries and farms. A cost-effective biosecurity program for farmed shrimp requires reliable diagnostic tools to make timely decisions to control or exclude pathogens. 

Stocking the best quality shrimp postlarvae, healthy and free of pathogens, is a critical management step with significant effects on the production and profitability of a shrimp farm. 

DNA barcoding and nucleic acid sequencing technologies are important tools to build and maintain an identification library of aquacultured and other aquatic species that is accessible online for the scientific, commercial and regulatory communities. 

Accurate PCR testing of shrimp seedstock assures shrimp farmers that their seedstock is clean of major diseases. Ring tests verify that labs conducting PCR testing are obtaining correct, reliable and reproducible results when testing for specific shrimp diseases. 

La presencia de AHPND-Vibrio parahaemolyticus puede ser detectada tanto en muestras de ADN fecal como en caldo bacteriano enriquecido con muestras de caldo de enriquecimiento que muestran mayor sensibilidad. 

The presence of AHPND-Vibrio parahaemolyticus can be detected both in fecal DNA samples and in the enriched bacterial broth with samples of enrichment broth showing increased sensitivity. 

本文介绍了新兴对虾微孢子虫病（EHP）的组织学特征，使用了对患病对虾肝胰腺组织，排泄物，养殖水缸中水样和一些卤虫生物量的PCR化验分析的方法来检测到EHP的存在。(Editor's note: This article was originally published on the Advocate in English on April 15, 2016.) 

The prevalence of Perkinsus sp. in cultured Pacific oysters and the infection intensity during an annual culture cycle using two detection methods were studied in Sinaloa, Mexico. Pathogen prevalence varied depending on the temperature and the age of the culture. 

Authors examined the histological features from shrimp infected with the emerging microsporidian parasite Enterocytozoon hepatopenaei (EHP). A PCR assay method was used to detected in hepatopancreatic tissue, feces and water sampled from infected shrimp tanks, and in some samples of Artemia biomass. 

In analyzing the plasmid sequence from the whole genome sequences of AHPND V. parahaemolyticus (V.p) isolates, researchers identified a clear geographical variation within the plasmid, and developed PCR methods to characterize AHPND V.p isolates as either Mexico-type or Southeast Asia-type. 

A study confirmed the utility for improved polymerase chain reaction (PCR) detection of the Vibrio bacteria that cause acute hepatopancreatic necrosis disease (AHPND) in asymptomatic shrimp by permitting the shrimp to expire and decompose for several hours prior to preservation and PCR processing.